Chinese Journal of Rice Science ›› 2013, Vol. 27 ›› Issue (2): 184-190.DOI: 10.3969/j.issn.10017216.2013.02.011

• Research Papers • Previous Articles     Next Articles

Resistance of Echinochloa crusgalli (L.) Beauv. to  Quinclorac in the Rice Growing region of the  Middle and Lower Reaches of Yangtze River  in China

MA Guolan 1,2, BAI  Lianyang 1,4,* , LIU  Ducai 2 , LIU Xueyuan 2 , YU  Liuqing 3,*   

  1. 1 College of Biosafety Science and Technology, Hunan Agricultural University, Changsha 410125, China; 2 Hunan Plant Protection Research Institute, Changsha 410125, China; 3 China National Rice Research Institute, Hangzhou 310006, China; 4 Hunan University of Humanities, Science and Technology, Loudi 417000, China; 
  • Received:2012-08-01 Revised:2012-09-26 Online:2013-03-10 Published:2013-03-10
  • Contact: BAI Lianyang1,4,*,YU Liuqing3,*

我国长江中下游稻区稗草对二氯喹啉酸的抗药性研究

马国兰1,2,柏连阳1,4,* ,刘都才 2 ,刘雪源2,余柳青 3,*   

  1. 1湖南农业大学 生物安全科学技术学院,  湖南 长沙  410125; 2湖南省植物保护研究所, 湖南 长沙  410125; 3中国水稻研究所, 浙江 杭州 310006; 4湖南人文科技学院, 湖南 娄底 417000;
  • 通讯作者: 柏连阳1,4,*,余柳青3,*
  • 基金资助:

    国家水稻产业技术体系资助项目 (CARS01); 国家自然科学基金资助项目(31201530); 国家公益性行业科研专项(201303031)。

Abstract: The resistance of Echinochloa crusgalli (L.) Beauv. to quinclorac was determined by using pot planting method with  42 biotypes collected from the rice growing  regions in the  middle and lower reaches  of  the Yangtze River in  China from 2008 to 2011.The results showed that higher resistance levels were observed in two biotypes of E. crusgalli from    Yiyang County (R1) and Wangcheng County (R2), Hunan Province with  the resistance indexes (RI)  of 21.84 and 32.31fold, respectively.  Other biotypes of E. crusgalli. from Hunan, Jiangsu and Zhejiang Province have developed lower level resistance (1.12-7.32fold). The 1aminocyclopropane1carboxylate synthase (ACS) activity in the  two resistance biotypes of E. crusgalli (R1 and R2)  declined significantly   one day after  quinclorac treatment, with  the RIs of  0.49 and 0.56fold compared with the contemporary contro1. Then the activity  increased slightly two days after treatment and  remained to be  lower than   the control; however  the activity  decreased slowly to 0.34 and 0.35fold relative to the control at  four days after treatment  The βcyanoalanine (βCAS) activity in R1 and R2 of E. crusgalli increased significantly after treated with  quinclorac; the maximum were observed five days after treatment,  being 1.80 and 2.05fold, as high as  the control, respectively.

Key words: Echinochloa crusgalli (L.) Beauv.; quinclorac; resistance, mechanism

摘要: 2008至2011年,采集了我国长江中下游稻区的42个稗草\[Echinochloa crusgalli (L.) Beauv.\]生物型,采用盆栽法测定了其对二氯喹啉酸的抗药性。结果表明, 湖南省益阳县(R1)和望城县(R2)的稗草生物型的抗性水平最高,分别达到21.84倍和32.31倍;浙江、江苏以及湖南省其他地区稗草生物型的抗性指数均在1.12~7.32倍,抗性较弱。经二氯喹啉酸处理后1  d,上述两个抗性生物型稗草(R1、R2)的1氨基环丙烷1羧酸合成酶(ACS)活性明显下降,为对照的49%和56%;2 d后小幅回升,并维持在对照之下,然后继续下降,处理4 d后下降至对照的34%和35%。经二氯喹啉酸处理后,R1和R2的β氰丙氨酸合成酶(βCAS)活性上升; 处理5 d后达到最高值,分别为对照的1.80倍和2.05倍。

关键词: 稗草, 二氯喹啉酸, 抗药性, 机理

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